个人主页：//bioli.cn E-mail：[email protected]
李田，博士，研究员，博士生导师
尊龙凯时-人生就是搏
西南大学微孢子虫感染与防控重庆市重点尊龙凯时
简历：
2021 - 至今，西南大学家蚕基因组生物学国家重点尊龙凯时，研究员，博士生导师；
2014 - 2021，西南大学家蚕基因组生物学国家重点尊龙凯时，副研究员，硕士生导师；
2014 - 2015，美国加州大学圣地亚哥分校访问学者；
2009 - 2014，西南大学蚕学与系统生物学研究所，讲师；
2005 - 2009，西南大学，博士，生物化学与分子生物学专业；
2003 - 2005，西南大学，硕士，微生物学专业；
2001 - 2003，西南农业大学，本科，计算机科学与技术专业；
1999 - 2003，西南农业大学，本科，动物医学专业。
招收研究生专业与方向:
1. 学术学位
071000 - 生物学
071005 - 微生物学
方向1: 微生物基因功能与应用
方向2: 微生物与宿主及环境互作
071010 - 生物化学与分子生物学
方向1: 基因组与生物信息学
方向2: 基因功能研究
090500 - 畜牧学
090504 - 特种动物科学
方向: 蚕桑病原生物学及病害防控
2. 专业学位
085238 - 生物工程领域
专业学位类别: 生物与医药
科研领域与方向:
主要以人兽共患微孢子虫和昆虫微孢子虫及其宿主(小鼠、家蚕等)为材料，从事基因组与生物信息学、病原侵染机制、宿主应答机制、流行病学、检测防治等研究。具体主要包括：
1. 微孢子虫感染与防控研究
筛选鉴定微孢子虫侵染致病关键因子和宿主应答关键因子，解析病原垂直传播的过程与分子机制，及其对宿主免疫、代谢和发育等重要通路的调控作用和机理，以及宿主免疫等系统应答感染的机制，并基于发现的重要靶标开展检测防控研究。
2. 基因组学与生物信息学研究
（1）开展病原微生物及宿主的基因组、转录组、蛋白质组、宏基因组等组学测序分析，解析病原流行特征与规律、侵染机制、宿主应答机制等重要问题，并为检测防控研究提供候选靶标。
（2）开发生物大数据与数据库平台 (BioDB Platform, //biodb.org)，研发生物信息分析方法和工具，构建生物信息数据库：qPrimerDB (Quantitative PCR Primer Database), MicroEpiDB (Microsporidia Epidemiology Database), MorusDB (Mulberry genome database), BrassicaEDB (Brassica Gene Expression Database), ... ...
主持科研项目：
◎ 国家自然科学基金面上项目, 54万元, 2023 - 2026;
◎ 教育部中央高校基本业务费先导计划团队项目, 60万元, 2023 - 2026;
◎ 国家自然科学基金面上项目, 62万元, 2018 - 2021;
◎ 国家自然科学基金面上项目, 80万元, 2015 - 2018;
◎ 教育部中央高校基本业务费创新团队项目, 50万元, 2015 - 2017;
◎ 国家自然科学基金青年基金项目 , 20万元, 2010 - 2013;
◎ 重庆市自然科学基金一般项目, 3万元, 2010 - 2013;
◎ 教育部中央高校基本业务费一般项目, 5万元, 2010 - 2012。
主要科研成果：
◎ 在PLoS Pathogens、Nucleic Acids Research、Parasites & Vectors、BMC Genomics等国内外期刊发表研究论文80余篇，参编专著和译著3部；开展了微孢子虫基因组、转录组、代谢组和蛋白质组等多组学测序与大数据分析，解析了微孢子虫经卵垂直传播、抑制宿主细胞凋亡、调控宿主线粒体片段化和相关重要通路的分子机制，搭建了生物大数据与数据库平台（BioDB Platform, //biodb.org），构建了代表性物种qPCR引物数据库(qPrimerDB)、微孢子虫流行病学数据库(MicroEpiDB)、家蚕病原数据库(SilkPathDB)、家蚕细胞器蛋白质组数据库(SilkOrganPDB)、桑树基因组数据库(MorusDB)和多杀性巴氏杆菌基因组数据库(PamulDB)等多个数据库；获得2017年度教育部高等学校科学研究优秀成果奖自然科学二等奖 (第三完成人)。
代表性论著：
28. Chunxia Wang†, Bin Yu†, Xianzhi Meng†, Dan Xia†, Boyan Pei, Xiangyou Tang, Guizheng Zhang, Junhong Wei, Mengxian Long, Jie Chen, Jialing Bao, Chunfeng Li, Guoqing Pan, Zeyang Zhou*, Tian Li*. Microsporidian Nosema bombycis hijacks host vitellogenin and restructures ovariole cells for transovarial transmission. PLoS Pathog. 2023, 19(12):e1011859. doi: 10.1371/journal.ppat.1011859. [PubMed] [PLoS Pathogens]
27. Tian Li, Junhong Wei, Guoqing Pan. Advances in the Genetic Manipulation of Nosema bombycis. Exp Suppl. 2022, 114:137-152. doi: 10.1007/978-3-030-93306-7_6. [Full Text]
26. Jian Luo, Jinzhi Xu, Chaolu Xie, Zuoming Zhao, Junrui Guo, Yuan Wen, Tian Li*, Zeyang Zhou*. Microsporidia promote host mitochondrial fragmentation by modulating DRP1 phosphorylation. Int. J. Mol. Sci. 2022, 23(14), 7746. DOI: 10.3390/ijms23147746. [ Full Text ] [PubMed]
25. Jinzhi Xu, Jian Luo, Jiajing Chen, Charles R. Vossbrinck, Tian Li* and Zeyang Zhou*. Characterization of the Largest Secretory Protein Family, Ricin B Lectin-like Protein, in Nosema bombycis: Insights into Microsporidian Adaptation to Host. Journal of Fungi, 2022, 8(6), 551. [Full Text]
24. Yinze Han, Hailong Gao, Jinzhi Xu, Jian Luo, Bin Yu, Chunxia Wang, Boyan Pei, Jiajing Chen, Bing Han, Tian Li*, Zeyang Zhou. Microsporidian Encephalitozoon hellem secretes EhPTP4 to regulate host endoplasmic reticulum-associated degradation. Acta Microbiologica Sinica, 2022, 62(1): 1–17. [DOI: 10.13343/j.cnki.wsxb.20210263] [Full Text]
23. Boyan Pei, Chunxia Wang, Bin Yu, Dan Xia, Tian Li* and Zeyang Zhou*. The First Report on the Transovarial Transmission of Microsporidian Nosema bombycis in Lepidopteran Crop Pests Spodoptera litura and Helicoverpa armigera. Microorganisms. 2021, 9(7): 1442. DOI: 10.3390/microorganisms9071442. [PubMed]
22. Tian Li*,†, Chen Xu†, Jinzhi Xu, Jian Luo, Bin Yu, Xianzhi Meng, Chunfeng Li, Guoqing Pan and Zeyang Zhou*. Proteomic Identification of Bombyx mori Organelles Using the Engineered Ascorbate Peroxidase APEX and Development of Silkworm Organelle Proteome Database (SilkOrganPDB). Int. J. Mol. Sci. 2021, 22(9):5051. DOI: 10.3390/ijms22095051. [PubMed]
21. Jian Luo, Qiang He, Jin-Zhi Xu, Chen Xu, Yin-Ze Han, Hai-Long Gao, Xian-Zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. Microsporidia infection upregulates host energy metabolism but maintains ATP homeostasis. J Invertebr Pathol. 2021, 107596. [PubMed]
20. Yingfei Ruan, Xiaofei Xu, Qiang He, Li Li, Junrui Guo, Jialing Bao, Guoqing Pan, Tian Li* and Zeyang Zhou. The largest meta-analysis on the global prevalence of microsporidia in mammals, avian and water provides insights into the epidemic features of these ubiquitous pathogens. Parasites & Vectors. 2021, 14:186. [PubMed]
19. Yinze Han, Hailong Gao, Jinzhi Xu, Jian Luo, Bing Han, Jialing Bao, Guoqing Pan, Tian Li*, Zeyang Zhou*. Innate and adaptive immune responses against microsporidia infection in mammals. Front. Microbiol.. 2020, 11:1468. [PubMed]
18. Qiang He, Jian Luo, Jin-Zhi Xu, Xian-Zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. In-vitro cultivation of Nosema bombycis sporoplasms: a method for potential genetic engineering of microsporidia. J Invertebr Pathol. 2020 Jun 6;174:107420. DOI: 10.1016/j.jip.2020.107420. [PubMed]
17. Qiang He†, Jian Luo†, Jin-Zhi Xu, Xian-zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. Characterization of Hsp70 gene family provides insight into its functions related to microsporidian proliferation. J Invertebr Pathol. 2020, 107394. DOI: 10.1016/j.jip.2020.107394. [PubMed]
16. Qiang He, Jian Luo, Jin-Zhi Xu, Chun-xia Wang, Xian-zhi Meng, Guo-Qing Pan, Tian Li*, Ze-Yang Zhou*. Morphology and Transcriptome Analysis of Nosema bombycis Sporoplasm and Insights into the Initial Infection of Microsporidia. mSphere. 2020 Feb 12;5(1). pii: e00958-19. DOI: 10.1128/mSphere.00958-19. [PubMed] [mSphere]
15. Tian Li*, Xiao-Fei Xu, Hui-Hui Du, Li Li, Neng-Zhang Li, Ze-Yang Zhou and Yuan-Yi Peng*. PamulDB: a comprehensive genomic resource for the study of human- and animal-pathogenic Pasteurella multocida. Database, 2019, baz025. DOI: 10.1093/database/baz025. [PubMed]
14. Qiang He, Charles R. Vossbrinck, Qiong Yang, Xian-Zhi Meng, Jian Luo, Guo-Qing Pan, Ze-Yang Zhou*, Tian Li*. Evolutionary and functional studies on microsporidian ATP-binding cassettes: Insights into the adaptation of microsporidia to obligated intracellular parasitism. Infect Genet Evol, 2019, 68: 136-144. DOI: 10.1016/j.meegid.2018.12.022. [PubMed]
13. Xian-Zhi Meng, Bo Luo, Xiang-You Tang, Qiang He, Ting-Rong Xiong, Zhuo-Ya Fang, Guoqing Pan, Tian Li*, Ze-Yang Zhou*. Pathological analysis of silkworm infected by two microsporidia Nosema bombycis CQ1 and Vairimorpha necatrix BM. J Invertebr Pathol. 2018, 153: 75-84. DOI: 10.1016/j.jip.2017.12.005. [PubMed]
12. Kun Lu†, Tian Li†, Jian He†, Wei Chang†, Rui Zhang, Miao Liu, Mengna Yu, Yonghai Fan, Jinqi Ma, Wei Sun, Cunmin Qu, Liezhao Liu, Nannan Li, Ying Liang, Rui Wang, Wei Qian, Zhanglin Tang, Xinfu Xu, Bo Lei, Kai Zhang, Jiana Li. qPrimerDB: a thermodynamics-based gene-specific qPCR primer database for 147 organisms. Nucleic Acids Research, 2017, gkx725. [PubMed] [PDF]. (†Authors contributed equally to this paper.)
11. Tian Li, Guo-Qing Pan, Charles R. Vossbrinck, Jin-Shan Xu, Chun-Feng Li, Jie Chen, Meng-Xian Long, Ming Yang, Xiao-Fei Xu, Chen Xu, Bettina A. Debrunner-Vossbrinck, Ze-Yang Zhou. SilkPathDB: a comprehensive resource for the study of silkworm pathogens. Database. 2017(1): bax001. [PubMed] [PDF]
10. 周泽扬，潘国庆†，李田†，许金山†.《家蚕微孢子虫基因组生物学》. 科学出版社. 2014, ISBN 978-7-03-042451-8. (†副主编).
9. Tian Li, Xiwu Qi, Qiwei Zeng, Zhonghuai Xiang, Ningjia He. MorusDB: A resource for mulberry genomics and genome biology. DATABASE. 2014, bau054, DOI: 10.1093/database/bau054. [PubMed]
8. Bo Luo, Handeng Liu, Guoqing Pan, Tian Li, Zeng Li, Xiaoqun Dang, Tie Liu, Zeyang Zhou. Morphological and molecular studies of Vairimorpha necatrix BM, a new strain of the microsporidium V. necatrix(microsporidia, burenellidae) recorded in the silkworm, Bombyx mori. Exp Parasitol. 2014, pii: S0014-4894(14)00115-5. DOI: 10.1016/j.exppara.2014.05.001. [PubMed]
7. He N, Zhang C, Qi X, Zhao S, Tao Y, Yang G, Lee TH, Wang X, Cai Q, Li D, Lu M, Liao S, Luo G, He R, Tan X, Xu Y, Li T, Zhao A, Jia L, Fu Q, Zeng Q, Gao C, Ma B, Liang J, Wang X, Shang J, Song P, Wu H, Fan L, Wang Q, Shuai Q, Zhu J, Wei C, Zhu-Salzman K, Jin D, Wang J, Liu T, Yu M, Tang C, Wang Z, Dai F, Chen J, Liu Y, Zhao S, Lin T, Zhang S, Wang J, Wang J, Yang H, Yang G, Wang J, Paterson AH, Xia Q, Ji D, Xiang Z. Draft genome sequence of the mulberry tree Morus notabilis. Nat Commun. 2013, 4:2445. DOI: 10.1038/ncomms3445.
6. LI Tian, QI Xiao-Ran, TAO Mei-Lin, LIU Xian-Lin, KANG Ding-Rong, QIN Guo-Wei, PAN Guo-Qing, ZHOU Ze-Yang. A Comparative Genomics Analysis of Secreted Proteins from Four Microsporidia. Science of Sericulture. 2013, 39(3): 0527-0536.
5. Guoqing Pan*, Jinshan Xu*, Tian Li*, Qingyou Xia, Shao-Lun Liu, Guojie Zhang, Songgang Li, Chunfeng Li, Handeng Liu, Liu Yang, Tie Liu, Xi Zhang, Zhengli Wu, Wei Fan, Xiaoqun Dang, Heng Xiang, Meilin Tao, Yanhong Li, Junhua Hu, Zhi Li, Lipeng Lin, Jie Luo, Lina Geng, LinLing Wang, Mengxia Long, Yongji Wan, Ningjia He, Ze Zhang, Cheng Lu, Patrick J Keeling, Jun Wang, Zhonghuai Xiang and Zeyang Zhou. Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation. BMC Genomics. 2013, 14: 186. (*Co-first authors).
4. LI Tian, LIU Xian-Lin, HAN Bing, QI Xiao-Ran, DANG Xiao-Qun, KANG Ding-Rong, PAN Guo-Qing, ZHOU Ze-Yang. A Genome-wide Prediction and Analysis on Secreted Proteins of Nosema bombycis. Science of Sericulture. 2013, 39(2): 0295-0301.
3. Tian Li, Xiaoqun Dang, Jinshan Xu, Handeng Liu, Guoqing Pan, Zeyang Zhou. Mitochondrial Pyruvate Dehydrogenase E1 of Nosema bombycis - a Marker in Microsporidian Evolution. Current Zoology. 2009, 55: 423-429.
2. LI Tian, PAN Guo-Qing, DANG Xiao-Qun, ZHOU Ze-Yang. cDNA Library Construction and EST Analysis of Bombyx mori Silk Gland Heavily Infected byNosema bombycis. Science of Sericulture. 2009, 35(2): 320-327.
1. Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, et al. A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science. 2004, 306: 1937-1940.